Comments on: What is SIRT1? Potential Activators, Inhibitors & Genetics

By: Andreia

Andreia — Mon, 24 Jun 2019 15:11:56 +0000

Sirt 1 promotes thyroid carcinogenesis! https://www.ncbi.nlm.nih.gov/pubmed/22986535

By: Matthew

Matthew — Wed, 17 Jan 2018 12:58:01 +0000

Fantastic article Joe :)

As someone who deals with such issues, I would like to emphasise the point that SIRT1 activation upregulates the MAO-A enzyme in the brain. MAO-A is critical in the breakdown of monoamines, such as serotonin, dopamine and histamine. Therefore, natural SIRT1 activators, as listed here, may well prove neurologically beneficial for those dealing with excess levels of monoamines.

By: Michelle Madsen

Michelle Madsen — Fri, 08 Sep 2017 16:43:44 +0000

Joe, two questions, do you believe in NAD supplements like Niagen (oral availability)? Second this observation, I have 3 times taken Niagen by itself…wicked headache which lasted 2 days each time – adequate break between trials for washout so I am certain. BUT I had previously taken Elysium Basis product, with is NR + the Pterostilbene component, no headache. I also routinely get a headache from Phosphatidylserine also, which is also a multi-day recovery event. any thoughts?

By: Phil Alcoceli

Phil Alcoceli — Wed, 30 Aug 2017 00:22:33 +0000

The problem with Niagen being short lived on the duration of its effects is solved by Life Extensions’ “Optimized NAD+ Cell Regenerator with Resveratrol”. If you want even better effects combine it with their “Liver Efficiency” supplement which increases SOD. Take one of each once a day away from food. Best of health to you!

By: Albert Radulescu

Albert Radulescu — Tue, 23 May 2017 13:24:05 +0000

I study medical nutrition in London and this website is gold, amazing !!!

By: Tara

Tara — Wed, 08 Mar 2017 00:46:59 +0000

In reply to wut. Oral NAD is poorly absorbed, which might be the reason you didn't feel anything. IV NAD therapy has the best absorption rate of NAD into the cells because you are able to get higher concentrations into the blood stream than oral supplementation. Are you looking to boost NAD levels for bio-hacking, or for a chronic condition?

By: Tara

Tara — Wed, 08 Mar 2017 00:43:43 +0000

In reply to Kyle. I know alcohol seriously impedes NAD recycling and production because it uses up a tremendous amount of NAD+ to metabolize ethanol. In fact, chronic alcohol use inhibits SIRT3, or maybe SIRT1, and enhances PARP1- which can signal cell death.

By: Tara

Tara — Wed, 08 Mar 2017 00:39:42 +0000

In reply to Dan.

Hi Dan,
You should look into IV NAD therapy. I know The NAD Treatment Center in San Diego has treated patients before with CFS with great success. Wish you the best of luck!
Tara

By: th

th — Thu, 16 Jun 2016 04:21:36 +0000

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257644/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840337/

grape seed extract/pycnogenol and nad+

By: David Bathalovski

David Bathalovski — Mon, 30 May 2016 00:58:04 +0000

I took H2 tablets with Niagen powder, oh my goodness. I can walk, talk, and have become a BRAND again. Saved my life after I took Cipro which lead to an explosion in my mercury levels. I was Cipro toxic and metal toxic. I am back. My brain is back.

By: john seville

john seville — Wed, 13 Apr 2016 16:25:42 +0000

In reading Vince Giuliano’s blog, his experience and observation in using NR or any other means of increasing NAD+ is transient at best, lasting only 2 weeks for him and apparently this also appears common to many reviews on sites such as Amazon from users of Niagen etc. As a user yourself of these compounds what is your opinion and any methods you believe would allow someone to continue to reap the rewards of increased NAD+ past an iniitial surge. Thanks in advance!

By: Joseph M. Cohen

Joseph M. Cohen — Sun, 13 Mar 2016 03:07:08 +0000

In reply to Leo. Yup, nicotinamide suppresses

By: Leo

Leo — Sun, 13 Mar 2016 01:23:03 +0000

Main differences in Nicotinamide vs Nicotinamide riboside? One suppresses SIRT1 at high dosage and the other one doesn’t?

By: brycemfsellers

brycemfsellers — Tue, 19 Jan 2016 18:44:17 +0000

In reply to Dan. Hi dan can u give us an update on how ur doing with lens therapy? i also did lens neurofeedback and found it very helpful! -bryce

By: Dylan

Dylan — Wed, 06 Jan 2016 22:21:58 +0000

Do you think taking leptin mimicing agents (stimulants/bromocriptine etc.) would nullify the modulatory effect eating/fasting has on NAD+?

By: ky

ky — Mon, 04 Jan 2016 08:46:47 +0000

In reply to Joseph M. Cohen.

I also found p53(tumor supressor, senescence inducer) level is decreased in mild hypoxia while increase in severe hypoxia. But, these studies was also performed in carcinoma cell lines. Can it be applied to normal cells?
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2745664/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2676422/

By: ky

ky — Mon, 04 Jan 2016 08:29:38 +0000

In reply to Joseph M. Cohen.

Hi, I look through the detail of above mentioned paper to figure out relation between sirt1 and hif, and found out there is not clear mechanism of co-relation, yet too complex.
You mentioned about ratio of NAD+/NADH, but it is not the only factor to influence the level of sirt1 expression.
“Although most hypoxia/pyridine nucleotide studies examined total NAD+ or NADH levels rather than free NAD+ or NADH levels or ratios, which are the relevant molecular species for NAD+/NADH-regulated enzymes such as Sirt1, it is nevertheless unlikely that a major change in direction of the cellular redox state during hypoxia would occur if free NAD+/free NADH ratios were calculated. Therefore, an alternative mechanism for activation of Sirt1 during hypoxia, besides changes in redox state and pyridine nucleotide levels/ratios, must exist” (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3077588/, Rui C, Hypoxia Increases Sirtuin 1 Expression in a Hypoxia-inducible Factor-dependent Manner)
Author mentions some possible pathways to explain mechanisms, but it is still unclear.
I am not sure if this study extends to normal cell lines. (The study was performed in carcinoma cell lines, which have telomerase activities while most normal cells do not have).
Do you know any papers regarding how sirt1 and hypoxic are related in normall cells?

By: Joseph M. Cohen

Joseph M. Cohen — Mon, 28 Dec 2015 16:38:24 +0000

In reply to ky. Thanks!

By: ky

ky — Mon, 28 Dec 2015 09:09:46 +0000

http://www.ncbi.nlm.nih.gov/pubmed/21345792
http://www.sciencemag.org/content/324/5932/1289.abstract
Still not sure of association between sirt1 expression and hif-1,2 expression.
Perhaps, because the relationship does not specify the concentration of oxygens.
See this study. http://scialert.net/fulltext/?doi=jbs.2010.545.554&org=11
It somewhat mentions about how nrf2 responds to the oxygen insufficiency.
Also, we have known that mTor signalling depends on hypoxic condtion and exposure time.
(http://www.ncbi.nlm.nih.gov/pubmed/19147540)
You can see DAF-2 related genes modulate the lifespans of C. elegan (http://www.ncbi.nlm.nih.gov/pubmed/23419779)
And, this seems to be related to stress response pathways with oxygen availability.
Of course, hypoxic response can be bad in moderate to severe situation, and I don’t think that low oxygen level is good in a common sense. However, it is suggested to mention about the dose(oxygen saturation) given in experiments to better understand hormetic shape.

By: Joseph M. Cohen

Joseph M. Cohen — Sat, 03 Oct 2015 11:58:32 +0000

In reply to Dan. Depression isn't from Niagen...

By: Dan

Dan — Wed, 30 Sep 2015 04:42:13 +0000

Hi my names is Dan. I have found your info very helpful. I have been taking a product with Niagen in it at 250 mgs per serving. At first I felt great then after a few weeks I feel a little depressed. Not sure if it is too much dopamine being produced which can cause depression. Have you heard anyone relay this specific side effect?. I also take prelox, curcumin, PS, Feel a little shaky too. Maybe too much other neurotransmitters being produced. Will cut back on dose. I have CFS and a therapy called LENS therapy has made an enormous difference and I believe it will soon cure me. I have had CFS for 30 years and nothing has made such a difference with measurable and steady improvement. Helps to dis-entrain a stress pattern in your brain which causes slow EEG wave patterns that causes your central nervous system to go awry. Chronic or unrelenting stress either emotional ,physical or due to illness is usually how most people with CFS first become ill. I know this is how I first became ill. Check it out and look into it more. I will keep you posted on my future progress.

By: Joseph M. Cohen

Joseph M. Cohen — Tue, 29 Sep 2015 15:06:54 +0000

In reply to Kyle. I interviewed him a few months ago. Will publish.

By: Kyle

Kyle — Mon, 28 Sep 2015 02:26:58 +0000

Have you ever thought of having Steve Fowkes on for an interview. He seems to be incredibly knowledgable about supplements and chemistry. He also seems to be very willing to help people out.

He gets into NADH in this article and states:”The natural production of NADH (in the absence of alcohol) depends on lipoic acid, thiamine and riboflavin. Since lipoic acid and thiamine contain sulfur which may become bound to acetaldehyde, I wonder whether the use of alcohol compromises the very energy pathways it stimulates.” What are your thoughts on this?

http://www.ceri.com/alcohol.htm

By: Joseph M. Cohen

Joseph M. Cohen — Mon, 21 Sep 2015 12:39:03 +0000

In reply to locosoroso. Niagen gets absorbed well